top of page

Paper published in iScience!

Title: phoP maintains the environmental persistence and virulence of pathogenic bacteria in mechanically stressed desiccated droplets

Authors: Vishnu Hariharan, Atish Roy Chowdhury, Srinivas Rao S, Dipshikha Chakravortty & Saptarshi Basu

Congratulations to the team!

Abstract: Despite extensive studies on kinematic features of impacting drops, the effect of mechanical stress on desiccated bacteria-laden droplets remains unexplored. In the present study, we unveiled the consequences of the impaction of bacteria-laden droplets on solid surfaces and their subsequent desiccation on the virulence of an enteropathogen Salmonella Typhimurium (STM). The methodology elucidated the deformation, cell-cell interactions, adhesion energy, and roughness in bacteria induced by impact velocity and low moisture due to evaporation. Salmonella retrieved from the dried droplets were used to understand fomite-mediated pathogenesis. The impact velocity-induced mechanical stress deteriorated the in vitro viability of Salmonella. Interestingly, an uninterrupted bacterial proliferation was observed in macrophages at higher mechanical stress. Wild-type Salmonella under mechanical stress induced the expression of phoP while infecting macrophages. The inability of STM ΔphoP to grow in nutrient-rich dried droplets signifies the role of phoP in sensing the mechanical stress and maintaining the virulence of Salmonella.

7 views0 comments

Recent Posts

See All

The European Physical Journal Special Topics: Fluid-Fluid and Fluid-Soft Matter Interaction guest edited by Prof. Saptarshi Basu and Prof. Aloke Kumar is now online.

We have filed a patent titled "Depth from Defocus (DFD) technique for calibration-free particle size estimation" Team: Saini Jatin Rao, Shubham Sharma, Cameron Tropea, Saptarshi Basu

bottom of page